› 3jzd

X-ray diffraction
2.1Å resolution

Crystal structure of Putative alcohol dehedrogenase (YP_298327.1) from RALSTONIA EUTROPHA JMP134 at 2.10 A resolution

Released:
DOI: 10.2210/pdb3jzd/pdb
PDB entry 3jzd coloured by chain, front view.
PDB entry 3jzd coloured by chain, side view.
PDB entry 3jzd coloured by chain, top view.
Source organism: Cupriavidus pinatubonensis JMP134
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
3-oxoadipate + NAD(P)(+) = 2-maleylacetate + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 358 amino acids
Theoretical weight: 37.62 KDa
Source organism: Cupriavidus pinatubonensis JMP134
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46TQ1 (Residues: 1-357; Coverage: 100%)
Gene name: Reut_B4129
Sequence domains: Iron-containing alcohol dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
7 bound ligands:
Ligand CA 2 x CA
Ligand CL 4 x CL
Ligand PEG 3 x PEG
Ligand PG4 2 x PG4
Ligand P6G 2 x P6G
Ligand GOL 3 x GOL
Ligand PGE 1 x PGE
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P3121
Unit cell:
a: 143.841Å b: 143.841Å c: 169.011Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.21
Expression system: Escherichia coli

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