Function and Biology Details
Reaction catalysed:
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Glycosyl hydrolase, family 13, catalytic domain
- 1,4-alpha-glucan-branching enzyme, GlgB
- Immunoglobulin E-set
- Immunoglobulin-like fold
- Alpha-amylase/branching enzyme, C-terminal all beta
- Glycosyl hydrolase, all-beta
- Glycoside hydrolase superfamily
- Glycoside hydrolase, family 13, N-terminal
2 more domains
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
1,4-alpha-glucan branching enzyme GlgB (preferred)
PDBe Complex ID:
PDB-CPX-161832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,4-alpha-glucan branching enzyme GlgB
Molecule details ›
Chain: A
Length: 722 amino acids
Theoretical weight: 80.83 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 722 amino acids
Theoretical weight: 80.83 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P9WN45 (Residues: 10-731; Coverage: 99%)
Sequence domains:
- Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
- Alpha amylase, catalytic domain
- Alpha amylase, C-terminal all-beta domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 21-ID-G
Spacegroup:
P21212
Expression system: Escherichia coli BL21(DE3)
