› 3k1k

X-ray diffraction
2.15Å resolution

Green fluorescent protein bound to enhancer nanobody

Released:
DOI: 10.2210/pdb3k1k/pdb
PDB entry 3k1k coloured by chain, front view.
PDB entry 3k1k coloured by chain, side view.
PDB entry 3k1k coloured by chain, top view.
Source organisms:
Primary publication:
Modulation of protein properties in living cells using nanobodies.
Kirchhofer A, Helma J, Schmidthals K, Frauer C, Cui S, Karcher A, Pellis M, Muyldermans S, Casas-Delucchi CS, Cardoso MC, Leonhardt H, Hopfner KP, Rothbauer U
Nat Struct Mol Biol 17 133-8 (2010)
PMID: 20010839

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211203 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 27.02 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein
Enhancer Chains: C, D
Molecule details ›
Chains: C, D
Length: 123 amino acids
Theoretical weight: 13.76 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 10 x MSE
Ligand GYS 2 x GYS

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P4222
Unit cell:
a: 160.47Å b: 160.47Å c: 78.828Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.255
Expression system: Escherichia coli

Quick links

Citations

46 review citations

Nanobodies: natural single-domain antibodies.
Muyldermans S. (2013)
45 more

45 mentions without citation

Nanobodies: Chemical Functionalization Strategies and Intracellular Applications.
Schumacher et al. (2018)
44 more