› 3k1t

X-ray diffraction
1.9Å resolution

Crystal structure of Putative gamma-glutamylcysteine synthetase (YP_546622.1) from METHYLOBACILLUS FLAGELLATUS KT at 1.90 A resolution

Released:
DOI: 10.2210/pdb3k1t/pdb
PDB entry 3k1t coloured by chain, front view.
PDB entry 3k1t coloured by chain, side view.
PDB entry 3k1t coloured by chain, top view.
Source organism: Methylobacillus flagellatus KT
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172853 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--cysteine ligase GshA Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 48.9 KDa
Source organism: Methylobacillus flagellatus KT
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1GYA6 (Residues: 1-431; Coverage: 100%)
Gene name: Mfla_2516
Sequence domains: Glutamate-cysteine ligase
Structure domains: Glutamate-cysteine ligase, N-terminal domain

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand CL 2 x CL
Ligand GOL 9 x GOL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I23
Unit cell:
a: 175.72Å b: 175.72Å c: 175.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.145 0.16
Expression system: Escherichia coli

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