3k2e

X-ray diffraction
1.9Å resolution

Crystal structure of enoyl-(acyl-carrier-protein) reductase from Anaplasma phagocytophilum at 1.9A resolution

Released:
Source organism: Anaplasma phagocytophilum
Entry authors: Abendroth J, Staker B, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-173611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-[acyl-carrier-protein] reductase [NADH] Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 31.91 KDa
Source organism: Anaplasma phagocytophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2GKM8 (Residues: 1-275; Coverage: 100%)
Gene names: APH_0473, fabI
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 80Å b: 89.4Å c: 78.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.151 0.194
Expression system: Escherichia coli BL21(DE3)