› 3k2l

X-ray diffraction
2.36Å resolution

Crystal Structure of dual-specificity tyrosine phosphorylation regulated kinase 2 (DYRK2)

Released:
DOI: 10.2210/pdb3k2l/pdb
PDB entry 3k2l coloured by chain, front view.
PDB entry 3k2l coloured by chain, side view.
PDB entry 3k2l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of Down syndrome kinases, DYRKs, reveal mechanisms of kinase activation and substrate recognition.
Soundararajan M, Roos AK, Savitsky P, Filippakopoulos P, Kettenbach AN, Olsen JV, Gerber SA, Eswaran J, Knapp S, Elkins JM
Structure 21 986-96 (2013)
PMID: 23665168

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity tyrosine-phosphorylation-regulated kinase 2 Chain: A
Molecule details ›
Chain: A
Length: 429 amino acids
Theoretical weight: 49.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92630 (Residues: 146-552; Coverage: 68%)
Gene name: DYRK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand NA 1 x NA
Ligand CL 2 x CL
3 modified residues:
Ligand SEP 3 x SEP
Ligand TPO 1 x TPO
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P42212
Unit cell:
a: 84.285Å b: 84.285Å c: 148.505Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

DYRK1A, a Dosage-Sensitive Gene Involved in Neurodevelopmental Disorders, Is a Target for Drug Development in Down Syndrome.
Duchon et al. (2016)
16 more

12 mentions without citation

Dual-Specificity, Tyrosine Phosphorylation-Regulated Kinases (DYRKs) and cdc2-Like Kinases (CLKs) in Human Disease, an Overview.
Lindberg et al. (2021)
11 more