› 3k44

X-ray diffraction
2.1Å resolution

Crystal Structure of Drosophila melanogaster Pur-alpha

Released:
DOI: 10.2210/pdb3k44/pdb
PDB entry 3k44 coloured by chain, front view.
PDB entry 3k44 coloured by chain, side view.
PDB entry 3k44 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
X-ray structure of Pur-alpha reveals a Whirly-like fold and an unusual nucleic-acid binding surface.
Graebsch A, Roche S, Niessing D
Proc Natl Acad Sci U S A 106 18521-6 (2009)
PMID: 19846792

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine-rich binding protein-alpha, isoform A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 146 amino acids
Theoretical weight: 16.78 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q95RR6 (Residues: 40-185; Coverage: 53%)
Gene names: CG1507, Dmel\CG1507, Dmel_CG1507, PURalpha, Pur alpha, Pur-a, Pur-alpha, dPur alpha, pur-alpha, purA
Sequence domains: PurA ssDNA and RNA-binding protein
Structure domains: Secreted effector protein pipB2 fold

Ligands and Environments

2 bound ligands:
Ligand CL 8 x CL
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1, ESRF BEAMLINE ID14-4
Spacegroup: P2
Unit cell:
a: 63.168Å b: 62.544Å c: 64.479Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.223 0.222 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Small-angle scattering for structural biology--expanding the frontier while avoiding the pitfalls.
Jacques et al. (2010)
5 more

12 mentions without citation

PURA syndrome: clinical delineation and genotype-phenotype study in 32 individuals with review of published literature.
Reijnders et al. (2018)
11 more