› 3k48

X-ray diffraction
2.8Å resolution

Crystal structure of APRIL bound to a peptide

Released:
DOI: 10.2210/pdb3k48/pdb
PDB entry 3k48 coloured by chain, front view.
PDB entry 3k48 coloured by chain, side view.
PDB entry 3k48 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Multiple novel classes of APRIL-specific receptor-blocking peptides isolated by phage display.
Gordon NC, Lien S, Johnson J, Wallweber HJ, Tran T, Currell B, Mathieu M, Quan C, Starovasnik MA, Hymowitz SG, Kelley RF
J Mol Biol 396 166-77 (2010)
PMID: 19945466

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163700 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor ligand superfamily member 13 Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 140 amino acids
Theoretical weight: 15.71 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9D777 (Residues: 104-241; Coverage: 57%)
Gene names: April, Tnfsf13
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
peptide Chains: R, S, T
Molecule details ›
Chains: R, S, T
Length: 16 amino acids
Theoretical weight: 2.03 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 100.811Å b: 84.771Å c: 55.562Å
α: 90° β: 99.45° γ: 90°
R-values:
R R work R free
0.212 0.205 0.267
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Peptide phage display as a tool for drug discovery: targeting membrane receptors.
Molek et al. (2011)
1 more