› 3k4b

X-ray diffraction
1.9Å resolution

Pyranose 2-oxidase T169S mutant

Released:
DOI: 10.2210/pdb3k4b/pdb
PDB entry 3k4b coloured by chain, front view.
PDB entry 3k4b coloured by chain, side view.
PDB entry 3k4b coloured by chain, top view.
Source organism: Trametes ochracea
Primary publication:
A conserved active-site threonine is important for both sugar and flavin oxidations of pyranose 2-oxidase.
Pitsawong W, Sucharitakul J, Prongjit M, Tan TC, Spadiut O, Haltrich D, Divne C, Chaiyen P
J Biol Chem 285 9697-9705 (2010)
PMID: 20089849

Function and Biology Details

Reaction catalysed: 
D-glucose + O(2) = 2-dehydro-D-glucose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyranose 2-oxidase Chain: A
Molecule details ›
Chain: A
Length: 623 amino acids
Theoretical weight: 69.4 KDa
Source organism: Trametes ochracea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7ZA32 (Residues: 1-623; Coverage: 100%)
Gene name: p2o
Sequence domains: GMC oxidoreductase
Structure domains: Phage tail proteins - 2 layer sandwich fold

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P42212
Unit cell:
a: 101.95Å b: 101.95Å c: 127.626Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.271
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Lignin degradation: microorganisms, enzymes involved, genomes analysis and evolution.
Janusz et al. (2017)
1 more

1 mention without citation

A conserved active-site threonine is important for both sugar and flavin oxidations of pyranose 2-oxidase.
Pitsawong et al. (2010)