› 3k4x

X-ray diffraction
2.98Å resolution

Eukaryotic Sliding Clamp PCNA Bound to DNA

Released:
DOI: 10.2210/pdb3k4x/pdb
PDB entry 3k4x coloured by chain, front view.
PDB entry 3k4x coloured by chain, side view.
PDB entry 3k4x coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Analysis of the role of PCNA-DNA contacts during clamp loading.
McNally R, Bowman GD, Goedken ER, O'Donnell M, Kuriyan J
BMC Struct Biol 10 3 (2010)
PMID: 20113510

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108168 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Proliferating cell nuclear antigen Chain: A
Molecule details ›
Chain: A
Length: 798 amino acids
Theoretical weight: 88.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A6ZL36 (Residues: 1-257, 2-257, 2-258; Coverage: 100%)
Gene names: POL30, SCY_0306
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
DNA (5'-D(*CP*CP*CP*AP*TP*CP*GP*TP*AP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 2.98 KDa
DNA (5'-D(*TP*TP*TP*TP*AP*TP*AP*CP*GP*AP*TP*GP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.33 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 83.935Å b: 93.712Å c: 136.883Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.28
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Proliferating cell nuclear antigen (PCNA): a key factor in DNA replication and cell cycle regulation.
Strzalka et al. (2011)
18 more

14 mentions without citation

Archaeal genome guardians give insights into eukaryotic DNA replication and damage response proteins.
Shin et al. (2014)
13 more