› 3k62

X-ray diffraction
1.9Å resolution

Crystal structure of FBF-2/gld-1 FBEb complex

Released:
DOI: 10.2210/pdb3k62/pdb
PDB entry 3k62 coloured by chain, front view.
PDB entry 3k62 coloured by chain, side view.
PDB entry 3k62 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Structural basis for specific recognition of multiple mRNA targets by a PUF regulatory protein.
Wang Y, Opperman L, Wickens M, Hall TM
Proc Natl Acad Sci U S A 106 20186-91 (2009)
PMID: 19901328

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170772 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Fem-3 mRNA-binding factor 2 Chain: A
Molecule details ›
Chain: A
Length: 412 amino acids
Theoretical weight: 46.96 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09312 (Residues: 164-575; Coverage: 65%)
Gene names: F21H12.5, fbf-2
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
5'-R(*UP*GP*UP*GP*UP*UP*AP*UP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.81 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P61
Unit cell:
a: 96.62Å b: 96.62Å c: 101.678Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.158 0.197
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

PUF proteins: repression, activation and mRNA localization.
Quenault et al. (2011)
12 more

2 mentions without citation

Structural basis for specific recognition of multiple mRNA targets by a PUF regulatory protein.
Wang et al. (2009)
1 more