› 3k92

X-ray diffraction
2.3Å resolution

Crystal structure of a E93K mutant of the majour Bacillus subtilis glutamate dehydrogenase RocG

Released:
DOI: 10.2210/pdb3k92/pdb
PDB entry 3k92 coloured by chain, front view.
PDB entry 3k92 coloured by chain, side view.
PDB entry 3k92 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Functional dissection of a trigger enzyme: mutations of the bacillus subtilis glutamate dehydrogenase RocG that affect differentially its catalytic activity and regulatory properties.
Gunka K, Newman JA, Commichau FM, Herzberg C, Rodrigues C, Hewitt L, Lewis RJ, Stülke J
J Mol Biol 400 815-27 (2010)
PMID: 20630473

Function and Biology Details

Reaction catalysed: 
L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153912 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catabolic NAD-specific glutamate dehydrogenase RocG Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 424 amino acids
Theoretical weight: 46.69 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P39633 (Residues: 1-424; Coverage: 100%)
Gene names: BSU37790, gudA, ipa-75d, rocG, yweB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PEG 5 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 137.614Å b: 143.072Å c: 162.607Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.238
Expression system: Escherichia coli

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Citations

6 review citations

Bacterial virulence in the moonlight: multitasking bacterial moonlighting proteins are virulence determinants in infectious disease.
Henderson et al. (2011)
5 more

2 mentions without citation

Multiple Forms of Glutamate Dehydrogenase in Animals: Structural Determinants and Physiological Implications.
Bunik et al. (2016)
1 more