› 3k96

X-ray diffraction
2.1Å resolution

2.1 Angstrom resolution crystal structure of glycerol-3-phosphate dehydrogenase (gpsA) from Coxiella burnetii

Released:
DOI: 10.2210/pdb3k96/pdb
PDB entry 3k96 coloured by chain, front view.
PDB entry 3k96 coloured by chain, side view.
PDB entry 3k96 coloured by chain, top view.
Source organism: Coxiella burnetii RSA 493
Entry authors: Minasov G, Halavaty A, Shuvalova L, Dubrovska I, Winsor J, Peterson SN, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
sn-glycerol 3-phosphate + NAD(P)(+) = glycerone phosphate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol-3-phosphate dehydrogenase [NAD(P)+] Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 38.86 KDa
Source organism: Coxiella burnetii RSA 493
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83BJ0 (Residues: 1-332; Coverage: 100%)
Gene names: CBU_1518, gpsA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EPE 2 x EPE
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 85.272Å b: 88.452Å c: 97.289Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.164 0.21
Expression system: Escherichia coli

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