› 3k9v

X-ray diffraction
2.5Å resolution

Crystal structure of rat mitochondrial P450 24A1 S57D in complex with CHAPS

Released:
DOI: 10.2210/pdb3k9v/pdb
PDB entry 3k9v coloured by chain, front view.
PDB entry 3k9v coloured by chain, side view.
PDB entry 3k9v coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of CYP24A1, a mitochondrial cytochrome P450 involved in vitamin D metabolism.
Annalora AJ, Goodin DB, Hong WX, Zhang Q, Johnson EF, Stout CD
J Mol Biol 396 441-51 (2010)
PMID: 19961857

Function and Biology Details

Reaction catalysed: 
Calcidiol + 2 reduced adrenodoxin + 2 H(+) + O(2) = secalciferol + 2 oxidized adrenodoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-170764 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 482 amino acids
Theoretical weight: 55.93 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09128 (Residues: 34-514; Coverage: 94%)
Gene names: Cyp24, Cyp24a1
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand CPS 6 x CPS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 182.609Å b: 81.647Å c: 108.699Å
α: 90° β: 122.89° γ: 90°
R-values:
R R work R free
0.209 0.206 0.252
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Vitamin D: Metabolism, Molecular Mechanism of Action, and Pleiotropic Effects.
Christakos et al. (2016)
19 more

21 mentions without citation

Cytochrome P450-mediated metabolism of vitamin D.
Jones et al. (2014)
20 more