› 3kbc

X-ray diffraction
3.51Å resolution

Crystal structure of GltPh K55C-A364C mutant crosslinked with divalent mercury

Released:
DOI: 10.2210/pdb3kbc/pdb
PDB entry 3kbc coloured by chain, front view.
PDB entry 3kbc coloured by chain, side view.
PDB entry 3kbc coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Transport mechanism of a bacterial homologue of glutamate transporters.
Reyes N, Ginter C, Boudker O
Nature 462 880-5 (2009)
PMID: 19924125

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate transporter homolog Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 425 amino acids
Theoretical weight: 44.89 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O59010 (Residues: 1-425; Coverage: 100%)
Gene name: PH1295
Sequence domains: Sodium:dicarboxylate symporter family
Structure domains: Sodium:dicarboxylate symporter

Ligands and Environments

3 bound ligands:
Ligand ASP 3 x ASP
Ligand HG 3 x HG
Ligand NA 6 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 113.142Å b: 206.78Å c: 205.971Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.267 0.27
Expression system: Escherichia coli

Quick links

Citations

54 review citations

Structural Biology of the Major Facilitator Superfamily Transporters.
Yan N. (2015)
53 more

55 mentions without citation

Structural perspectives on secondary active transporters.
Boudker et al. (2010)
54 more