› 3kc6

X-ray diffraction
2.05Å resolution

Crystal structure of the large c-terminal domain of polymerase basic protein 2 from influenza virus a/viet nam/1203/2004 (h5n1)

Released:
DOI: 10.2210/pdb3kc6/pdb
PDB entry 3kc6 coloured by chain, front view.
PDB entry 3kc6 coloured by chain, side view.
PDB entry 3kc6 coloured by chain, top view.
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Primary publication:
Biological and structural characterization of a host-adapting amino acid in influenza virus.
Yamada S, Hatta M, Staker BL, Watanabe S, Imai M, Shinya K, Sakai-Tagawa Y, Ito M, Ozawa M, Watanabe T, Sakabe S, Li C, Kim JH, Myler PJ, Phan I, Raymond A, Smith E, Stacy R, Nidom CA, Lank SM, Wiseman RW, Bimber BN, O'Connor DH, Neumann G, Stewart LJ, Kawaoka Y
PLoS Pathog 6 e1001034 (2010)
PMID: 20700447

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 22.77 KDa
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6DNN3 (Residues: 538-741; Coverage: 27%)
Gene name: PB2
Sequence domains:
Structure domains: Polymerase Basic Protein 2, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 33.084Å b: 65.696Å c: 96.855Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

Viral quasispecies evolution.
Domingo et al. (2012)
39 more

9 mentions without citation

Biological and structural characterization of a host-adapting amino acid in influenza virus.
Yamada et al. (2010)
8 more