› 3kcp

X-ray diffraction
1.94Å resolution

Crystal structure of interacting Clostridium thermocellum multimodular components

Released:
DOI: 10.2210/pdb3kcp/pdb
PDB entry 3kcp coloured by chain, front view.
PDB entry 3kcp coloured by chain, side view.
PDB entry 3kcp coloured by chain, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Primary publication:
Insights into higher-order organization of the cellulosome revealed by a dissect-and-build approach: crystal structure of interacting Clostridium thermocellum multimodular components.
Adams JJ, Currie MA, Ali S, Bayer EA, Jia Z, Smith SP
J Mol Biol 396 833-9 (2010)
PMID: 20070943

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107055 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cellulosomal-scaffolding protein A Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 34.66 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06851 (Residues: 1542-1853; Coverage: 17%)
Gene names: Cthe_3077, cipA
Sequence domains:
Structure domains:
SLH domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 187 amino acids
Theoretical weight: 20.57 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DF10 (Residues: 27-200; Coverage: 28%)
Gene name: Cthe_1307
Sequence domains: Cohesin domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21212
Unit cell:
a: 169.977Å b: 58.019Å c: 56.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.231
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Insights into cellulosome assembly and dynamics: from dissection to reconstruction of the supramolecular enzyme complex.
Smith et al. (2013)
2 more

4 mentions without citation

Enhanced sampling techniques in molecular dynamics simulations of biological systems.
Bernardi et al. (2015)
3 more