› 3kcu

X-ray diffraction
2.24Å resolution

Structure of formate channel

Released:
DOI: 10.2210/pdb3kcu/pdb
PDB entry 3kcu coloured by chain, front view.
PDB entry 3kcu coloured by chain, side view.
PDB entry 3kcu coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel.
Wang Y, Huang Y, Wang J, Cheng C, Huang W, Lu P, Xu YN, Wang P, Yan N, Shi Y
Nature 462 467-72 (2009)
PMID: 19940917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142234 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate channel FocA Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 285 amino acids
Theoretical weight: 31.01 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC25 (Residues: 1-285; Coverage: 100%)
Gene names: ECs0987, Z1250, focA
Sequence domains: Formate/nitrite transporter
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

1 bound ligand:
Ligand MA5 1 x MA5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 101.378Å b: 107.934Å c: 163.128Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.232
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Structural Biology of the Major Facilitator Superfamily Transporters.
Yan N. (2015)
20 more

22 mentions without citation

Inverted topologies in membrane proteins: a mini-review.
Duran et al. (2013)
21 more