› 3kd9

X-ray diffraction
2.75Å resolution

Crystal structure of pyridine nucleotide disulfide oxidoreductase from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb3kd9/pdb
PDB entry 3kd9 coloured by chain, front view.
PDB entry 3kd9 coloured by chain, side view.
PDB entry 3kd9 coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Entry authors: Agarwal R, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
2 CoA + NADP(+) = CoA-disulfide + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)H coenzyme A polysulfide/persulfide reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 449 amino acids
Theoretical weight: 49.54 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58308 (Residues: 7-444; Coverage: 98%)
Gene name: PH0572
Sequence domains:
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 114.81Å b: 114.81Å c: 353.917Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.284
Expression system: Escherichia coli BL21(DE3)

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