› 3kdf

X-ray diffraction
1.98Å resolution

X-ray Crystal Structure of the Human Replication Protein A Complex from Wheat Germ Cell Free Expression

Released:
DOI: 10.2210/pdb3kdf/pdb
PDB entry 3kdf coloured by chain, front view.
PDB entry 3kdf coloured by chain, side view.
PDB entry 3kdf coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Burgie ES, Bingman CA, Phillips Jr GN, Fox BG, Makino S-I, Center for Eukaryotic Structural Genomics (CESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147543 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Replication protein A 14 kDa subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 121 amino acids
Theoretical weight: 13.77 KDa
Source organism: Homo sapiens
Expression system: cell-free synthesis
UniProt:
  • Canonical: P35244 (Residues: 1-121; Coverage: 100%)
Gene names: REPA3, RPA14, RPA3
Sequence domains: Replication factor A protein 3
Structure domains: Nucleic acid-binding proteins
Replication protein A 32 kDa subunit Chains: B, D
Molecule details ›
Chains: B, D
Length: 132 amino acids
Theoretical weight: 14.98 KDa
Source organism: Homo sapiens
Expression system: cell-free synthesis
UniProt:
  • Canonical: P15927 (Residues: 41-172; Coverage: 49%)
Gene names: REPA2, RPA2, RPA32, RPA34
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

1 bound ligand:
Ligand EDO 7 x EDO
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 66.68Å b: 77.7Å c: 121.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.206
Expression system: cell-free synthesis

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