› 3kds

X-ray diffraction
2.6Å resolution

apo-FtsH crystal structure

Released:
DOI: 10.2210/pdb3kds/pdb
PDB entry 3kds coloured by chain, front view.
PDB entry 3kds coloured by chain, side view.
PDB entry 3kds coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
The crystal structure of apo-FtsH reveals domain movements necessary for substrate unfolding and translocation.
Bieniossek C, Niederhauser B, Baumann UM
Proc Natl Acad Sci U S A 106 21579-84 (2009)
PMID: 19955424

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent zinc metalloprotease FtsH Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 465 amino acids
Theoretical weight: 51.41 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WZ49 (Residues: 147-610; Coverage: 76%)
Gene names: TM_0580, ftsH
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand NHX 3 x NHX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P622
Unit cell:
a: 190.5Å b: 190.5Å c: 152.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

AAA+ proteases: ATP-fueled machines of protein destruction.
Sauer et al. (2011)
13 more

11 mentions without citation

Mechanistic insights into bacterial AAA+ proteases and protein-remodelling machines.
Olivares et al. (2016)
10 more