› 3kew

X-ray diffraction
2Å resolution

Crystal structure of probable alanyl-trna-synthase from Clostridium perfringens

Released:
DOI: 10.2210/pdb3kew/pdb
PDB entry 3kew coloured by chain, front view.
PDB entry 3kew coloured by chain, side view.
PDB entry 3kew coloured by chain, top view.
Source organism: Clostridium perfringens ATCC 13124
Entry authors: Patskovsky Y, Toro R, Gilmore M, Miller S, Sauder JM, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonyl/alanyl tRNA synthetase SAD domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 27.86 KDa
Source organism: Clostridium perfringens ATCC 13124
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2YPJ4 (Residues: 2-231; Coverage: 58%)
Gene name: CPF_0714
Sequence domains: Threonyl and Alanyl tRNA synthetase second additional domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 57.849Å b: 57.922Å c: 178.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.259
Expression system: Escherichia coli

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