› 3kfl

X-ray diffraction
2Å resolution

Leishmania major methionyl-tRNA synthetase in complex with methionyladenylate and pyrophosphate

Released:
DOI: 10.2210/pdb3kfl/pdb
PDB entry 3kfl coloured by chain, front view.
PDB entry 3kfl coloured by chain, side view.
PDB entry 3kfl coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Structure of Leishmania major methionyl-tRNA synthetase in complex with intermediate products methionyladenylate and pyrophosphate.
Larson ET, Kim JE, Zucker FH, Kelley A, Mueller N, Napuli AJ, Verlinde CL, Fan E, Buckner FS, Van Voorhis WC, Merritt EA, Hol WG
Biochimie 93 570-82 (2011)
PMID: 21144880

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
methionine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 564 amino acids
Theoretical weight: 63.98 KDa
Source organism: Leishmania major
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4QCD2 (Residues: 206-747; Coverage: 73%)
Gene name: LMJF_21_0810
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand ME8 1 x ME8
Ligand POP 1 x POP
Ligand MG 1 x MG
Ligand ZN 1 x ZN
Ligand EDO 7 x EDO
Ligand FMT 4 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 68.816Å b: 88.802Å c: 101.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Aminoacyl-tRNA synthetases as drug targets in eukaryotic parasites.
Pham et al. (2014)
5 more

13 mentions without citation

Copaifera of the Neotropics: A Review of the Phytochemistry and Pharmacology.
da Trindade et al. (2018)
12 more