› 3kg4

X-ray diffraction
1.95Å resolution

Crystal structure of an uncharacterized protein from Mannheimia succiniciproducens

Released:
DOI: 10.2210/pdb3kg4/pdb
PDB entry 3kg4 coloured by chain, front view.
PDB entry 3kg4 coloured by chain, side view.
PDB entry 3kg4 coloured by chain, top view.
Source organism: [Mannheimia] succiniciproducens MBEL55E
Entry authors: Bonanno JB, Dickey M, Bain KT, Lau C, Romero R, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR pre-crRNA endoribonuclease Cas5d Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 27.09 KDa
Source organism: [Mannheimia] succiniciproducens MBEL55E
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q65TW5 (Residues: 2-225; Coverage: 100%)
Gene names: MS0988, cas5d
Sequence domains: CRISPR-associated protein (Cas_Cas5)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P63
Unit cell:
a: 81.827Å b: 81.827Å c: 61.937Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.211 0.242
Expression system: Escherichia coli BL21(DE3)

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