› 3kg9

X-ray diffraction
1.7Å resolution

Dehydratase domain from CurK module of Curacin polyketide synthase

Released:
DOI: 10.2210/pdb3kg9/pdb
PDB entry 3kg9 coloured by chain, front view.
PDB entry 3kg9 coloured by chain, side view.
PDB entry 3kg9 coloured by chain, top view.
Source organism: Lyngbya majuscula
Primary publication:
Crystal structures of dehydratase domains from the curacin polyketide biosynthetic pathway.
Akey DL, Razelun JR, Tehranisa J, Sherman DH, Gerwick WH, Smith JL
Structure 18 94-105 (2010)
PMID: 20152156

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CurK Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 32.71 KDa
Source organism: Lyngbya majuscula
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6DNE2 (Residues: 958-1250; Coverage: 13%)
Gene name: curK
Sequence domains:
Structure domains: Polyketide synthase dehydratase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 38.122Å b: 94.301Å c: 150.989Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.217
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The structures of type I polyketide synthases.
Keatinge-Clay AT. (2012)
14 more

8 mentions without citation

Active site comparisons and catalytic mechanisms of the hot dog superfamily.
Labonte et al. (2013)
7 more