› 3kgy

X-ray diffraction
1.5Å resolution

Crystal structure of Putative dihydrofolate reductase (YP_001636057.1) from CHLOROFLEXUS AURANTIACUS J-10-FL at 1.50 A resolution

Released:
DOI: 10.2210/pdb3kgy/pdb
PDB entry 3kgy coloured by chain, front view.
PDB entry 3kgy coloured by chain, side view.
PDB entry 3kgy coloured by chain, top view.
Source organism: Chloroflexus aurantiacus J-10-fl
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial bifunctional deaminase-reductase C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 26.6 KDa
Source organism: Chloroflexus aurantiacus J-10-fl
Expression system: Escherichia coli
UniProt:
  • Canonical: A9WHX7 (Residues: 1-212; Coverage: 100%)
Gene name: Caur_2460
Sequence domains: RibD C-terminal domain
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

3 bound ligands:
Ligand NDP 2 x NDP
Ligand EDO 18 x EDO
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 55.231Å b: 57.352Å c: 140.979Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.174
Expression system: Escherichia coli

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