› 3khi

X-ray diffraction
1.95Å resolution

Crystal structure of a Putative Metal-dependent Hydrolase (YP_001336084.1) from Klebsiella pneumoniae subsp. pneumoniae MGH 78578 at 1.95 A resolution

Released:
DOI: 10.2210/pdb3khi/pdb
PDB entry 3khi coloured by chain, front view.
PDB entry 3khi coloured by chain, side view.
PDB entry 3khi coloured by chain, top view.
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Primary publication:
The structure of Mlc titration factor A (MtfA/YeeI) reveals a prototypical zinc metallopeptidase related to anthrax lethal factor.
Xu Q, Göhler AK, Kosfeld A, Carlton D, Chiu HJ, Klock HE, Knuth MW, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Jahreis K, Wilson IA
J Bacteriol 194 2987-99 (2012)
PMID: 22467785

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108065 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mlc titration factor A Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 30.67 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Expression system: Escherichia coli
UniProt:
  • Canonical: A6TB83 (Residues: 1-265; Coverage: 100%)
Gene names: KPN78578_23930, KPN_02432, mtfA, yeeI
Sequence domains: Glucose-regulated metallo-peptidase M90
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 2 x CL
Ligand EDO 5 x EDO
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P41212
Unit cell:
a: 131.19Å b: 131.19Å c: 37.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.213
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Architecture and function of metallopeptidase catalytic domains.
Cerdà-Costa et al. (2014)
2 more

2 mentions without citation

The structure of Mlc titration factor A (MtfA/YeeI) reveals a prototypical zinc metallopeptidase related to anthrax lethal factor.
Xu et al. (2012)
1 more