› 3kke

X-ray diffraction
2.2Å resolution

Crystal structure of a LacI family transcriptional regulator from Mycobacterium smegmatis

Released:
DOI: 10.2210/pdb3kke/pdb
PDB entry 3kke coloured by chain, front view.
PDB entry 3kke coloured by chain, side view.
PDB entry 3kke coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Entry authors: Bonanno JB, Rutter M, Bain KT, Do J, Ozyurt S, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lacI-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 303 amino acids
Theoretical weight: 32.02 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0QPR6 (Residues: 50-341; Coverage: 85%)
Gene name: MSMEG_0491
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand ACT 5 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 64.599Å b: 65.468Å c: 116.034Å
α: 90° β: 90.72° γ: 90°
R-values:
R R work R free
0.197 0.194 0.247
Expression system: Escherichia coli BL21(DE3)

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