› 3klk

X-ray diffraction
1.65Å resolution

Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form

Released:
DOI: 10.2210/pdb3klk/pdb
PDB entry 3klk coloured by chain, front view.
PDB entry 3klk coloured by chain, side view.
PDB entry 3klk coloured by chain, top view.
Source organism: Limosilactobacillus reuteri
Primary publication:
Crystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymes.
Vujicic-Zagar A, Pijning T, Kralj S, López CA, Eeuwema W, Dijkhuizen L, Dijkstra BW
Proc Natl Acad Sci U S A 107 21406-11 (2010)
PMID: 21118988

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dextransucrase Chain: A
Molecule details ›
Chain: A
Length: 1039 amino acids
Theoretical weight: 116.74 KDa
Source organism: Limosilactobacillus reuteri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SBN3 (Residues: 742-1772; Coverage: 60%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 58.279Å b: 65.949Å c: 82.506Å
α: 73.3° β: 78.46° γ: 85.82°
R-values:
R R work R free
0.167 0.165 0.195
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies.
Schmid et al. (2015)
11 more

17 mentions without citation

Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes.
Meng et al. (2016)
16 more