› 3klz

X-ray diffraction
2.5Å resolution

Pentameric formate channel with formate bound

Released:
DOI: 10.2210/pdb3klz/pdb
PDB entry 3klz coloured by chain, front view.
PDB entry 3klz coloured by chain, side view.
PDB entry 3klz coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Structure and mechanism of a pentameric formate channel.
Waight AB, Love J, Wang DN
Nat Struct Mol Biol 17 31-7 (2010)
PMID: 20010838

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192059 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate transporter 1, putative Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 280 amino acids
Theoretical weight: 30.58 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KRE7 (Residues: 1-280; Coverage: 100%)
Gene name: VC_1695
Sequence domains: Formate/nitrite transporter
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

2 bound ligands:
Ligand BOG 14 x BOG
Ligand FMT 39 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 99.779Å b: 100.456Å c: 192.955Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.223
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Common folds and transport mechanisms of secondary active transporters.
Shi Y. (2013)
8 more

5 mentions without citation

Structure and mechanism of a pentameric formate channel.
Waight et al. (2010)
4 more