› 3kre

X-ray diffraction
1.8Å resolution

Crystal structure of phosphoribosylaminoimidazole-succinocarboxamide synthase from Ehrlichia chaffeensis at 1.8A resolution

Released:
DOI: 10.2210/pdb3kre/pdb
PDB entry 3kre coloured by chain, front view.
PDB entry 3kre coloured by chain, side view.
PDB entry 3kre coloured by chain, top view.
Source organism: Ehrlichia chaffeensis str. Arkansas
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-173594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 30.37 KDa
Source organism: Ehrlichia chaffeensis str. Arkansas
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2GHH2 (Residues: 1-242; Coverage: 100%)
Gene names: ECH_0290, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand EDO 10 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 75.96Å b: 61.92Å c: 68.2Å
α: 90° β: 117.99° γ: 90°
R-values:
R R work R free
0.155 0.153 0.19
Expression system: Escherichia coli BL21(DE3)

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