› 3ks4

X-ray diffraction
2.4Å resolution

Crystal structure of Reston ebolavirus VP35 RNA binding domain

Released:
DOI: 10.2210/pdb3ks4/pdb
PDB entry 3ks4 coloured by chain, front view.
PDB entry 3ks4 coloured by chain, side view.
PDB entry 3ks4 coloured by chain, top view.
Source organism: Reston ebolavirus - Reston
Primary publication:
Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression.
Kimberlin CR, Bornholdt ZA, Li S, Woods VL, MacRae IJ, Saphire EO
Proc Natl Acad Sci U S A 107 314-9 (2010)
PMID: 20018665

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase cofactor VP35 Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 20.57 KDa
Source organism: Reston ebolavirus - Reston
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8JPY0 (Residues: 160-329; Coverage: 52%)
Gene names: REBOVgp2, VP35
Sequence domains: Filoviridae VP35
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R32
Unit cell:
a: 158.16Å b: 158.16Å c: 166.11Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.198 0.219
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Filovirus pathogenesis and immune evasion: insights from Ebola virus and Marburg virus.
Messaoudi et al. (2015)
24 more

6 mentions without citation

Hiding the evidence: two strategies for innate immune evasion by hemorrhagic fever viruses.
Hastie et al. (2012)
5 more