› 3ks8

X-ray diffraction
2.4Å resolution

Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA

Released:
DOI: 10.2210/pdb3ks8/pdb
PDB entry 3ks8 coloured by chain, front view.
PDB entry 3ks8 coloured by chain, side view.
PDB entry 3ks8 coloured by chain, top view.
Source organism: Reston ebolavirus - Reston
Primary publication:
Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression.
Kimberlin CR, Bornholdt ZA, Li S, Woods VL, MacRae IJ, Saphire EO
Proc Natl Acad Sci U S A 107 314-9 (2010)
PMID: 20018665

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185010 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Polymerase cofactor VP35 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 184 amino acids
Theoretical weight: 20.57 KDa
Source organism: Reston ebolavirus - Reston
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8JPY0 (Residues: 160-329; Coverage: 52%)
Gene names: REBOVgp2, VP35
Sequence domains: Filoviridae VP35
Structure domains:
5'-R(*AP*GP*AP*AP*GP*GP*AP*GP*GP*GP*AP*GP*GP*GP*AP*GP*GP*A)-3' Chain: E
Molecule details ›
Chain: E
Length: 18 nucleotides
Theoretical weight: 6.06 KDa
Source organism: Reston ebolavirus - Reston
Expression system: Not provided
5'-R(*UP*CP*CP*UP*CP*CP*CP*UP*CP*CP*CP*UP*CP*CP*UP*UP*CP*U)-3' Chain: F
Molecule details ›
Chain: F
Length: 18 nucleotides
Theoretical weight: 5.46 KDa
Source organism: Reston ebolavirus - Reston
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P31
Unit cell:
a: 85.685Å b: 85.685Å c: 108.812Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.198 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

26 review citations

Filovirus pathogenesis and immune evasion: insights from Ebola virus and Marburg virus.
Messaoudi et al. (2015)
25 more

11 mentions without citation

Host-pathogen protein-nucleic acid interactions: A comprehensive review.
Jain et al. (2022)
10 more