› 3kss

X-ray diffraction
3.88Å resolution

Structure and Mechanism of the Heavy Metal Transporter CusA

Released:
DOI: 10.2210/pdb3kss/pdb
PDB entry 3kss coloured by chain, front view.
PDB entry 3kss coloured by chain, side view.
PDB entry 3kss coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structures of the CusA efflux pump suggest methionine-mediated metal transport.
Long F, Su CC, Zimmermann MT, Boyken SE, Rajashankar KR, Jernigan RL, Yu EW
Nature 467 484-8 (2010)
PMID: 20865003

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cation efflux system protein CusA Chain: A
Molecule details ›
Chain: A
Length: 1055 amino acids
Theoretical weight: 115.83 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P38054 (Residues: 1-1047; Coverage: 100%)
Gene names: JW0564, b0575, cusA, ybdE
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

1 bound ligand:
Ligand CU1 1 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 180.363Å b: 180.363Å c: 287.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.263 0.26 0.325
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Copper toxicity and the origin of bacterial resistance--new insights and applications.
Dupont et al. (2011)
24 more

10 mentions without citation

Bacterial multidrug efflux transporters.
Delmar et al. (2014)
9 more