3kt8

X-ray diffraction
3Å resolution

Crystal structure of S. cerevisiae tryptophanyl-tRNA synthetase in complex with L-tryptophanamide

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP +diphosphate + H(+).
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-171295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan--tRNA ligase, cytoplasmic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 438 amino acids
Theoretical weight: 50.25 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12109 (Residues: 1-432; Coverage: 100%)
Gene names: HRE432, WRS1, YOL097C
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P61
Unit cell:
a: 252.26Å b: 252.26Å c: 111.376Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.247 0.273
Expression system: Escherichia coli BL21(DE3)