› 3kw7

X-ray diffraction
3.44Å resolution

Crystal structure of LacB from Trametes sp. AH28-2

Released:
DOI: 10.2210/pdb3kw7/pdb
PDB entry 3kw7 coloured by chain, front view.
PDB entry 3kw7 coloured by chain, side view.
PDB entry 3kw7 coloured by chain, top view.
Source organism: Trametes sp. AH28-2
Primary publication:
Structure of native laccase B from Trametes sp. AH28-2.
Ge H, Gao Y, Hong Y, Zhang M, Xiao Y, Teng M, Niu L
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 254-8 (2010)
PMID: 20208154

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Laccase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 502 amino acids
Theoretical weight: 53.9 KDa
Source organism: Trametes sp. AH28-2
UniProt:
  • Canonical: Q5I7J0 (Residues: 24-525; Coverage: 100%)
Gene name: lacB
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand CU 8 x CU
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P41
Unit cell:
a: 98.778Å b: 98.778Å c: 149.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.292

Quick links

Citations

1 citations in other articles

Construction and direct electrochemistry of orientation controlled laccase electrode.
Li et al. (2014)

8 mentions without citation

A highly efficient recombinant laccase from the yeast Yarrowia lipolytica and its application in the hydrolysis of biomass.
Kalyani et al. (2015)
7 more