Function and Biology Details
Reaction catalysed:
Preferred amino acids around the cleavage site can be denoted BBBBxHx-|-H, in which B denotes Arg or Lys, H denotes a hydrophobic amino acid, and x is any amino acid. The only known protein substrates are mitogen-activated protein (MAP) kinase kinases.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Bacterial exotoxin B
- Anthrax toxin, lethal/endema factor
- Protective antigen, heptamerisation domain
- Protective antigen, domain 4
- Clostridial binary toxin B/anthrax toxin PA, domain 3
- Protective antigen, heptamerisation domain superfamily
- Protective antigen, Ca-binding domain
- Anthrax toxin lethal factor-like domain
2 more domains
Structure analysis Details
Assembly composition:
hetero dodecamer (preferred)
Assembly name:
Protective antigen and Lethal factor (preferred)
PDBe Complex ID:
PDB-CPX-146589 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protective antigen PA-63
Molecule details ›
Chains: A, B, D, E
Length: 548 amino acids
Theoretical weight: 61.48 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 548 amino acids
Theoretical weight: 61.48 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P13423 (Residues: 197-764; Coverage: 75%)
Sequence domains:
- Clostridial binary toxin B/anthrax toxin PA Ca-binding domain
- Clostridial binary toxin B/anthrax toxin PA domain 2
- Clostridial binary toxin B/anthrax toxin PA domain 3
- Anthrax protective antigen, immunoglobulin-like domain
Lethal factor
Molecule details ›
Chains: C, F
Length: 263 amino acids
Theoretical weight: 30.55 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Anthrax toxin lethal factor, N- and C-terminal domain
Structure domains: Collagenase (Catalytic Domain)
Length: 263 amino acids
Theoretical weight: 30.55 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P15917 (Residues: 34-296; Coverage: 34%)
Sequence domains: Anthrax toxin lethal factor, N- and C-terminal domain
Structure domains: Collagenase (Catalytic Domain)
