› 3kyq

X-ray diffraction
2.44Å resolution

Lipid-induced Conformational Switch Controls Fusion Activity of Longin Domain SNARE Ykt6

Released:
DOI: 10.2210/pdb3kyq/pdb
PDB entry 3kyq coloured by chain, front view.
PDB entry 3kyq coloured by chain, side view.
PDB entry 3kyq coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Lipid-Induced conformational switch controls fusion activity of longin domain SNARE Ykt6.
Wen W, Yu J, Pan L, Wei Z, Weng J, Wang W, Ong YS, Tran TH, Hong W, Zhang M
Mol Cell 37 383-95 (2010)
PMID: 20159557

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Synaptobrevin homolog YKT6 Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 22.51 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5EGY4 (Residues: 1-198; Coverage: 100%)
Gene name: Ykt6
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand DPV 1 x DPV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2221
Unit cell:
a: 54.81Å b: 59.71Å c: 108.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.199 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

SNARE proteins in membrane trafficking.
Wang et al. (2017)
8 more

5 mentions without citation

A SNARE geranylgeranyltransferase essential for the organization of the Golgi apparatus.
Shirakawa et al. (2020)
4 more