› 3kz0

X-ray diffraction
2.35Å resolution

MCL-1 complex with MCL-1-specific selected peptide

Released:
DOI: 10.2210/pdb3kz0/pdb
PDB entry 3kz0 coloured by chain, front view.
PDB entry 3kz0 coloured by chain, side view.
PDB entry 3kz0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Determinants of BH3 binding specificity for Mcl-1 versus Bcl-xL.
Dutta S, Gullá S, Chen TS, Fire E, Grant RA, Keating AE
J Mol Biol 398 747-62 (2010)
PMID: 20363230

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163717 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Induced myeloid leukemia cell differentiation protein Mcl-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 158 amino acids
Theoretical weight: 17.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07820 (Residues: 172-327; Coverage: 45%)
Gene names: BCL2L3, MCL1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
Mcl-1 specific peptide MB7 Chains: C, D
Molecule details ›
Chains: C, D
Length: 23 amino acids
Theoretical weight: 2.8 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 7 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 64.325Å b: 121.523Å c: 46.379Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.27
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

BCL-2 family proteins: changing partners in the dance towards death.
Kale et al. (2018)
15 more

8 mentions without citation

Determinants of BH3 binding specificity for Mcl-1 versus Bcl-xL.
Dutta et al. (2010)
7 more