› 3kz4

X-ray diffraction
3.8Å resolution

Crystal Structure of the Rotavirus Double Layered Particle

Released:
DOI: 10.2210/pdb3kz4/pdb
PDB entry 3kz4 coloured by chain, front view.
PDB entry 3kz4 coloured by chain, side view.
PDB entry 3kz4 coloured by chain, top view.
Source organism: Bovine rotavirus strain UK/G6
Primary publication:
X-ray crystal structure of the rotavirus inner capsid particle at 3.8 A resolution.
McClain B, Settembre E, Temple BR, Bellamy AR, Harrison SC
J Mol Biol 397 587-99 (2010)
PMID: 20122940

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 900-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148072 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Inner capsid protein VP2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 880 amino acids
Theoretical weight: 102.6 KDa
Source organism: Bovine rotavirus strain UK/G6
Expression system: Chlorocebus sabaeus
UniProt:
  • Canonical: P17462 (Residues: 1-473, 474-881; Coverage: 100%)
Sequence domains: Rotavirus VP2 protein
Intermediate capsid protein VP6 Chains: C, D, E, F, G, H, I, J, K, L, M, N, O
Molecule details ›
Chains: C, D, E, F, G, H, I, J, K, L, M, N, O
Length: 397 amino acids
Theoretical weight: 44.92 KDa
Source organism: Bovine rotavirus strain UK/G6
Expression system: Chlorocebus sabaeus
UniProt:
  • Canonical: P18610 (Residues: 1-397; Coverage: 100%)
Sequence domains: Rotavirus major capsid protein VP6
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 740.75Å b: 1,198.07Å c: 1,345.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.328 0.328 0.339
Expression system: Chlorocebus sabaeus

Quick links

Citations

20 review citations

Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy.
Grigorieff et al. (2011)
19 more

19 mentions without citation

Structural insights into the coupling of virion assembly and rotavirus replication.
Trask et al. (2012)
18 more