› 3l0m

X-ray diffraction
3.45Å resolution

Crystal structure of Rab1-activation domain and P4M domain of SidM/DrrA from legionella

Released:
DOI: 10.2210/pdb3l0m/pdb
PDB entry 3l0m coloured by chain, front view.
PDB entry 3l0m coloured by chain, side view.
PDB entry 3l0m coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
Structural mechanism of host Rab1 activation by the bifunctional Legionella type IV effector SidM/DrrA.
Zhu Y, Hu L, Zhou Y, Yao Q, Liu L, Shao F
Proc Natl Acad Sci U S A 107 4699-704 (2010)
PMID: 20176951

Function and Biology Details

Reaction catalysed: 
GTP + [protein]-L-tyrosine = diphosphate + [protein]-O(4)-(5'-guanylyl)-L-tyrosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multifunctional virulence effector protein DrrA Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 38.33 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZSQ3 (Residues: 317-647; Coverage: 51%)
Gene names: drrA, lpg2464, sidM
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P41
Unit cell:
a: 144.337Å b: 144.337Å c: 102.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.241
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
17 more

4 mentions without citation

The unity of opposites: Strategic interplay between bacterial effectors to regulate cellular homeostasis.
Iyer et al. (2021)
3 more