› 3l1p

X-ray diffraction
2.8Å resolution

POU protein:DNA complex

Released:
DOI: 10.2210/pdb3l1p/pdb
PDB entry 3l1p coloured by chain, front view.
PDB entry 3l1p coloured by chain, side view.
PDB entry 3l1p coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A unique Oct4 interface is crucial for reprogramming to pluripotency.
Esch D, Vahokoski J, Groves MR, Pogenberg V, Cojocaru V, Vom Bruch H, Han D, Drexler HC, Araúzo-Bravo MJ, Ng CK, Jauch R, Wilmanns M, Schöler HR
Nat Cell Biol 15 295-301 (2013)
PMID: 23376973

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116512 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
POU domain, class 5, transcription factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 17.96 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20263 (Residues: 131-282; Coverage: 43%)
Gene names: Oct-3, Oct-4, Otf-3, Otf3, Pou5f1
Sequence domains:
Structure domains:
DNA (5'-D(*TP*CP*CP*AP*CP*AP*TP*TP*TP*GP*AP*AP*AP*GP*GP*CP*AP*AP*AP*TP*GP*GP*A)-3') Chain: N
Molecule details ›
Chain: N
Length: 23 nucleotides
Theoretical weight: 7.1 KDa
Source organism: Mus musculus
Expression system: Not provided
DNA (5'-D(*AP*TP*CP*CP*AP*TP*TP*TP*GP*CP*CP*TP*TP*TP*CP*AP*AP*AP*TP*GP*TP*GP*G)-3') Chain: M
Molecule details ›
Chain: M
Length: 23 nucleotides
Theoretical weight: 7.02 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41
Unit cell:
a: 49.25Å b: 49.25Å c: 265.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.257
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

27 review citations

Chromatin dynamics during cellular reprogramming.
Apostolou et al. (2013)
26 more

22 mentions without citation

Proteins Recognizing DNA: Structural Uniqueness and Versatility of DNA-Binding Domains in Stem Cell Transcription Factors.
Yesudhas et al. (2017)
21 more