› 3l1r

X-ray diffraction
3.2Å resolution

X-ray structure of the mutant lys300met of polyamine oxidase from zea mays in complex with spermidine

Released:
DOI: 10.2210/pdb3l1r/pdb
PDB entry 3l1r coloured by chain, front view.
PDB entry 3l1r coloured by chain, side view.
PDB entry 3l1r coloured by chain, top view.
Source organism: Zea mays
Entry authors: Fiorillo A, Ilari A, Tavladoraki P

Function and Biology Details

Reactions catalysed: 
Spermidine + O(2) + H(2)O = propane-1,3-diamine + 4-aminobutanal + H(2)O(2)
N(8)-acetylspermidine + O(2) + H(2)O = propane-1,3-diamine + 4-acetamidobutanal + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130269 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Polyamine oxidase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 478 amino acids
Theoretical weight: 54.53 KDa
Source organism: Zea mays
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O64411 (Residues: 29-500; Coverage: 100%)
Gene names: MPAO, MPAO1, PAO
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 1 x NAG
Ligand SPD 2 x SPD
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 137.676Å b: 137.676Å c: 189.148Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.192 0.216
Expression system: Komagataella pastoris

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