› 3l25

X-ray diffraction
2Å resolution

Crystal structure of Zaire Ebola VP35 interferon inhibitory domain bound to 8 bp dsRNA

Released:
DOI: 10.2210/pdb3l25/pdb
PDB entry 3l25 coloured by chain, front view.
PDB entry 3l25 coloured by chain, side view.
PDB entry 3l25 coloured by chain, top view.
Source organism: Ebola virus - Mayinga, Zaire, 1976
Primary publication:
Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35.
Leung DW, Prins KC, Borek DM, Farahbakhsh M, Tufariello JM, Ramanan P, Nix JC, Helgeson LA, Otwinowski Z, Honzatko RB, Basler CF, Amarasinghe GK
Nat Struct Mol Biol 17 165-72 (2010)
PMID: 20081868

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170144 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Polymerase cofactor VP35 Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 129 amino acids
Theoretical weight: 14.21 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05127 (Residues: 215-340; Coverage: 37%)
Gene name: VP35
Sequence domains: Filoviridae VP35
Structure domains:
RNA (5'-R(*CP*GP*CP*AP*UP*GP*CP*G)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 8 nucleotides
Theoretical weight: 2.54 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Not provided

Ligands and Environments

5 bound ligands:
Ligand MG 1 x MG
Ligand CL 4 x CL
Ligand NA 8 x NA
Ligand GOL 1 x GOL
Ligand FMT 24 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 78.947Å b: 84.571Å c: 89.316Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.207
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

35 review citations

Pattern Recognition and Signaling Mechanisms of RIG-I and MDA5.
Reikine et al. (2014)
34 more

17 mentions without citation

Filovirus pathogenesis and immune evasion: insights from Ebola virus and Marburg virus.
Messaoudi et al. (2015)
16 more