› 3l27

X-ray diffraction
1.95Å resolution

Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant

Released:
DOI: 10.2210/pdb3l27/pdb
PDB entry 3l27 coloured by chain, front view.
PDB entry 3l27 coloured by chain, side view.
PDB entry 3l27 coloured by chain, top view.
Source organism: Ebola virus - Mayinga, Zaire, 1976
Primary publication:
Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35.
Leung DW, Prins KC, Borek DM, Farahbakhsh M, Tufariello JM, Ramanan P, Nix JC, Helgeson LA, Otwinowski Z, Honzatko RB, Basler CF, Amarasinghe GK
Nat Struct Mol Biol 17 165-72 (2010)
PMID: 20081868

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase cofactor VP35 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 129 amino acids
Theoretical weight: 14.12 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05127 (Residues: 215-340; Coverage: 37%)
Gene name: VP35
Sequence domains: Filoviridae VP35
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CL 6 x CL
Ligand PO4 9 x PO4
Ligand GOL 10 x GOL
Ligand NA 1 x NA
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 81.549Å b: 81.549Å c: 343.493Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.176 0.229
Expression system: Escherichia coli

Quick links

Citations

39 review citations

Pattern Recognition and Signaling Mechanisms of RIG-I and MDA5.
Reikine et al. (2014)
38 more

3 mentions without citation

Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35.
Leung et al. (2010)
2 more