› 3l29

X-ray diffraction
1.7Å resolution

Crystal Structure of Zaire Ebola VP35 interferon inhibitory domain K319A/R322A mutant

Released:
DOI: 10.2210/pdb3l29/pdb
PDB entry 3l29 coloured by chain, front view.
PDB entry 3l29 coloured by chain, side view.
PDB entry 3l29 coloured by chain, top view.
Source organism: Ebola virus - Mayinga, Zaire, 1976
Primary publication:
Mutations abrogating VP35 interaction with double-stranded RNA render Ebola virus avirulent in guinea pigs.
Prins KC, Delpeut S, Leung DW, Reynard O, Volchkova VA, Reid SP, Ramanan P, Cárdenas WB, Amarasinghe GK, Volchkov VE, Basler CF
J Virol 84 3004-15 (2010)
PMID: 20071589

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase cofactor VP35 Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.07 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05127 (Residues: 215-340; Coverage: 37%)
Gene name: VP35
Sequence domains: Filoviridae VP35
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 51.43Å b: 66.07Å c: 72.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.232
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Viral tricks to grid-lock the type I interferon system.
Versteeg et al. (2010)
28 more

2 mentions without citation

Structural and functional characterization of Reston Ebola virus VP35 interferon inhibitory domain.
Leung et al. (2010)
1 more