› 3l2a

X-ray diffraction
1.71Å resolution

Crystal structure of Reston Ebola VP35 interferon inhibitory domain

Released:
DOI: 10.2210/pdb3l2a/pdb
PDB entry 3l2a coloured by chain, front view.
PDB entry 3l2a coloured by chain, side view.
PDB entry 3l2a coloured by chain, top view.
Source organism: Reston ebolavirus - Reston (1989)
Primary publication:
Structural and functional characterization of Reston Ebola virus VP35 interferon inhibitory domain.
Leung DW, Shabman RS, Farahbakhsh M, Prins KC, Borek DM, Wang T, Mühlberger E, Basler CF, Amarasinghe GK
J Mol Biol 399 347-57 (2010)
PMID: 20399790

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase cofactor VP35 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.35 KDa
Source organism: Reston ebolavirus - Reston (1989)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8JPY0 (Residues: 204-329; Coverage: 38%)
Gene names: REBOVgp2, VP35
Sequence domains: Filoviridae VP35
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACY 2 x ACY
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41
Unit cell:
a: 51.551Å b: 51.551Å c: 50.865Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.225
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Filovirus pathogenesis and immune evasion: insights from Ebola virus and Marburg virus.
Messaoudi et al. (2015)
10 more

3 mentions without citation

Hiding the evidence: two strategies for innate immune evasion by hemorrhagic fever viruses.
Hastie et al. (2012)
2 more