› 3l3q

X-ray diffraction
2.3Å resolution

Mouse importin alpha-pepTM NLS peptide complex

Released:
DOI: 10.2210/pdb3l3q/pdb
PDB entry 3l3q coloured by chain, front view.
PDB entry 3l3q coloured by chain, side view.
PDB entry 3l3q coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Probing the specificity of binding to the major nuclear localization sequence-binding site of importin-alpha using oriented peptide library screening.
Yang SN, Takeda AA, Fontes MR, Harris JM, Jans DA, Kobe B
J Biol Chem 285 19935-46 (2010)
PMID: 20406804

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156504 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 427 amino acids
Theoretical weight: 46.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 71-497; Coverage: 81%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
pepTM Chains: B, C
Molecule details ›
Chains: B, C
Length: 15 amino acids
Theoretical weight: 1.8 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 78.756Å b: 90.899Å c: 100.968Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.227
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Recognition of nuclear targeting signals by Karyopherin-β proteins.
Xu et al. (2010)
6 more

5 mentions without citation

Probing the specificity of binding to the major nuclear localization sequence-binding site of importin-alpha using oriented peptide library screening.
Yang et al. (2010)
4 more