› 3l4b

X-ray diffraction
3.45Å resolution

Crystal Structure of an Octomeric Two-Subunit TrkA K+ Channel Ring Gating Assembly, TM1088A:TM1088B, from Thermotoga maritima

Released:
DOI: 10.2210/pdb3l4b/pdb
PDB entry 3l4b coloured by chain, front view.
PDB entry 3l4b coloured by chain, side view.
PDB entry 3l4b coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Entry authors: Deller MC, Johnson HA, Miller M, Spraggon G, Wilson IA, Lesley SA, Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112517 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RCK N-terminal domain-containing protein Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 143 amino acids
Theoretical weight: 16.05 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: D3KFX7 (Residues: 1-143; Coverage: 100%)
Gene name: TM1088
Sequence domains: TrkA-N domain
Structure domains: NAD(P)-binding Rossmann-like Domain
Trk system potassium uptake protein TrkA Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 218 amino acids
Theoretical weight: 24.07 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: D3KFX8 (Residues: 1-218; Coverage: 100%)
Gene name: TM1088
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AMP 4 x AMP
Ligand UNX 1 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P3221
Unit cell:
a: 96.778Å b: 96.778Å c: 305.098Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.261 0.259 0.308
Expression system: Escherichia coli

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