› 3l60

X-ray diffraction
2Å resolution

Crystal structure of branched-chain alpha-keto acid dehydrogenase subunit e2 from mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb3l60/pdb
PDB entry 3l60 coloured by chain, front view.
PDB entry 3l60 coloured by chain, side view.
PDB entry 3l60 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Zencheck WD, Bonanno JB, Patskovsky Y, Toro R, Freeman J, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
2-methylpropanoyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-(2-methylpropanoyl)dihydrolipoyl)lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyllysine-residue acyltransferase component of branched-chain alpha-ketoacid dehydrogenase complex Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 26.68 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: O06159 (Residues: 154-393; Coverage: 61%)
Gene names: Rv2495c, bkdC, pdhC
Sequence domains: 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

1 bound ligand:
Ligand UNL 1 x UNL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I23
Unit cell:
a: 118.764Å b: 118.764Å c: 118.764Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.222
Expression system: Escherichia coli

Quick links